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A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. Springer Nature. Arruda LK, Vailes LD, Platts-Mills TAE, Fernandes-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspistz CK, Chapman MD: Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5. Animals sensitized and challenged with BtE showed higher levels of total cells and eosinophils in the BALF than control mice (p < 0.001, Figure 2A, and p < 0.01, Figure 2B; Tukey's test). Este texto é disponibilizado nos termos da licença. (D) Anti-Bt or anti-OVA IgG1 antibodies. The cell pellets were resuspended in PBS and lysed by three successive freezing and/thawing procedures, and then assayed for peroxidase activity in 96-well microassay plates, in duplicates, using 6.6 mM H2O2 and 1.5 mM orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). Am J Respir Crit Care Med 1999,160(4):1150–6. Neuza M Alcântara-Neves. statement and The following parameters were used to measure the immune response in mice of four inbred strains (CBA/J, BALB/c, A/J and C57Bl/6): (i) the total number of leukocytes and eosinophils in the bronchoalveolar lavage fluid (BALF); (ii) the concentration of IL-4 and IL-13 cytokines and eosinophil peroxidase (EPO) in the BALF; (iii) the serum levels of anti-B. (C) Level of eosinophil peroxidase (EPO) activity in BALF. Groups of mice from different mouse strains were sensitized to BtE by subcutaneous injections of 100 μg or 10 μg of BtE adsorbed to 1.6 mg of alum [Al(OH)3] gel (Sigma Chemical Co., St. Louis, MO, USA) on days 0 and 7 and challenged intranasally with 10 μg of BtE in 50 μl of saline on days 8, 10, 12 and 14. Castro Almarales RL, Mateo Morejon M, Naranjo Robalino RM, Navarro Viltre BI, Alvarez Castello M, Ronquillo Diaz M, Garcia Gomez I, Oliva Diaz Y, Gonzalez Leon M, Rodriguez Canosa JS, Labrada Rosado A: Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics. Experimental data indicate that inbred mouse strains differ in their ability to mount an allergen-induced asthmatic response [17, 18]. Eight to 10 week-old CBA/J, BALB/c, A/J and C57BL/6 male mice, and 3 to 4 month-old Wistar rats, were bred and maintained at the animal houses of the Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Brazil, and of the Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Hasegawa K, Tamari M, Shao C, Shimizu M, Takahashi N, Mao XQ, Yamasaki A, Kamada F, Doi S, Fujiwara H, Miyatake A, Fujita K, Tamura G, Matsubara Y, Shirakawa T, Suzuki Y: Variations in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma in human beings. Takeda F, Arakawa T, Toma H, Ishii A, Sato Y: Intranasal sensitization with Blomia tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum IgE and peripheral blood eosinophil counts. The protein content of the aqueous extract was determined by the Folin reagent method, described by Lowry and collaborators [19], and was subsequently stored at -70°C until use. Storage mites can also adapt their feeding to house dust. Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. CAS  Effect of immunization with 10 μg per injection ofB. In addition to allergen-dependent differences in intensity and nature of the allergic responses, the genetic makeup of the host seems to play an important role in murine models of respiratory allergy. Clin Exp Allergy 2002,32(11):1667–74. Sabit M, Ramos JDA, Alejandro GJ, and Gala C. Seasonal distribution of airborne pollen in Manila, Philippines, and the effect of meteorological factors to its daily concentrations.Aerobiologia 2015 (DOI 10.1007/s10453-015-9414-2). Olívia Flores, Candeias, Vitória da Conquista, Bahia, CEP 4503100, Brazil, Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo; Av. volume 11, Article number: 51 (2010) See more. Baqueiro, T., Russo, M., Silva, V.M. The effect of BtE and OVA sensitizations on total IgE and specific antibodies levels are shown in Figure 4. J Allergy Clin Immunol 1997,100(6 Pt 1):S2–24. PubMed Google Scholar. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE. Smith H: Animal models of asthma. Nat Immunol 2000,1(3):221–6. Article  A-D, Student's t test; E, Mann-Whitney's test. (F) IL-13 concentration in BALF. Cookies policy. Google Scholar. A familia Sarcoptidae inclúe animais parasitos como a carracha. Int Arch Allergy Immunol 2001,126(4):286–93. Rev Inst Med Trop Sao Paulo 2004,46(1):1–8. We also concluded that a murine experimental model of respiratory allergy that uses BtE as allergen differs quantitatively in immunological and pathological parameters when compared with the classical experimental model that uses OVA as allergen. Another hypothesis is that proteases present in the BtE cleave CD23, a negative regulator of IgE production [34]. Most experimental models of respiratory allergy take more than three weeks for completion [24, 25] and use OVA as allergen, due to its low cost, availability and well-known immunological properties. The mice were painlessly killed 24 h after the last allergen challenge. BtE-immunized mice of the most responsive strain (A/J strain) were then assessed for the presence of intra-bronchial mucous, airway hyperresponsiveness (AHR) to methacholine challenge and inflammatory cell infiltration in lung tissue. Animals that were not subcutaneously immunized with BtE (they received instead control injections of alum), and were subsequently challenged with BtE, did not differ from control, non-immunized mice that were challenged with saline, in any of the studied parameters (data not shown). Despite the bulk of work done in humans on mite-specific allergy, data on allergic responses to B. tropicalis antigens in murine models are scarce [14–16]. A/J mice were also considered the best responders to Dermatophagoides sp allergens among four studied strains [25]. Each symbol corresponds to the result obtained from an individual animal. On the other hand, OVA sensitization led to higher mucus production, and serum levels of specific IgE, IgG1 and IgG2a than BtE sensitization. Presence of mucus in bronchi of A/J mice sensitized with ovalbumin (OVA),Blomia tropicalisextract (Bt) or saline (Control). Life Sci 1969,15;8(16):813–20. Health issues Allergies . Notably, mice sensitized with BtE produced higher amounts of total IgE than those sensitized with OVA, in amounts similar to those observed with immunization with helminth antigens [32]. Google Scholar. (B) saline-injected group. In Europe, detectable Der p 1 or Der f 1 allergen was found in 68% of surveyed homes. Total cell counts were carried out using a Neubauer chamber. This is accordance to Takeda and collaborators' observation that the intranasal instillation alone of BtE elicited an IgE antibody response only when the antigen was continuously administered for a period of over 24 weeks [15]. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. http://creativecommons.org/licenses/by/2.0. 室塵 蟎 ( ㄇㄢˊ ) 屬(学名: Dermatophagoides )(英語: house dust mite )是一種8隻腳的微小動物,屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。. tropicalisextract (Bt E) on the development of experimental respiratory allergy in A/J mice. Int Arch Allergy Immunol 2003,132(4):346–54. Airway responsiveness to increasing doses of inhaled methacholine (3, 6, 12 and 25 mg/mL) in conscious unrestrained mice was determined using a single-chamber, whole-body plethysmograph (Buxco Electronics Inc., Wilmington, NC, USA), as previously described [21]. Asian Pac J Allergy Immunol 2002;20(4):217-21 . Lack of human IgE cross … Blomia tropicalis is a mite that belongs to the superfamily of Glycyphagidae. [11]. 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